Main Content Area
Physiology Faculty
Life Science II, Room 176
Email: ppatrylo@siumed.edu
- PHSL 410/511 Coordinator and Excitable Membrane Unit Lecturer
- Medical School Year One Curriculum Tutor and Resource Faculty (SSB unit)
A large fraction of my research program has focused on characterizing and understanding how alterations in neuronal connectivity/efficacy affect behavior (i.e., seizures and cognition). While my interest in this topic started during my graduate studies focusing on the characterization of hippocampal malformations in developmental mutant mouse strains, it really took off during my post-doctoral/research associate years (mentors: Drs. FE Dudek and Anne Williamson) which focused on animal models of temporal lobe epilepsy (TLE) as well as hippocampal tissue resected from patients with intractable TLE. Upon joining the faculty at Southern Illinois University School of Medicine (2001) I expanded this research program by incorporating additional models of epilepsy (cortical malformation related epilepsy) as well as aged rodents (models of "normal" and pathological aging - Alzheimer's disease) in order to begin to assess whether there could be common underlying alterations in synaptic connectivity/efficacy that could account for both a pro-convulsive phenotype and cognitive decline being that these two phenotypes are frequent co-morbidities.
During the last several years my laboratory has additionally begun to examine how metabolism/anti-oxidadants can affect synaptic efficacy/plasticity in “normal” and aberrant circuits and subsequently impair behavior. These experiments have primarily been performed in models of Alzheimer's disease and normal aging.
Patrylo PR, Sekiguchi M, and Nowakowski RS (1990) Heterozygote effects in dreher mice. J. Neurogenetics 6: 173-181.
Schweitzer JS, Patrylo PR, and Dudek FE (1992) Prolonged field bursts in the dentate gyrus: Dependence on low calcium, high potassium, and nonsynaptic mechanisms. J. Neurophysiol. 68: 2016-2025.
Patrylo PR and Nowakowski RS (1994) The morphology and distribution of astrocytes in the molecular layer of the dentate gyrus in NZB/BlNJ, dreher, and C57BL/6J mice. Glia 10: 1-9.
atrylo PR, Schweitzer JS, and Dudek FE (1994) Potassium-dependent prolonged field bursts in the dentate gyrus: Effects of extracellular calcium and amino acid receptor antagonists. Neuroscience 61(1): 13-19.
Patrylo PR, Kuhn AJ, Schweitzer JS, and Dudek FE (1996) Multiple-unit recordings during slow field-potential shifts in low-[Ca2+]o solutions in rat hippocampal and cortical slices. Neuroscience. 74: 107-118.
Patrylo PR and Dudek FE (1998) Physiological unmasking of new glutamatergic pathways in the dentate gyrus of hippocampal slices from epileptic kainate-treated rats. J. Neurophysiol. 22: 418-429.
Hellier J, Patrylo PR, Buckmaster PS, and Dudek FE (1998) Recurrent spontaneous motor seizures after repeated low-dose systemic treatment with kainate: Assessment of a rat model of temporal lobe epilepsy. Epilepsy Res. 31: 73-84.
van den Pol AN, Gao XB, Patrylo PR, Ghosh PK, and Obrietan K (1998) Glutamate inhibits GABA excitatory activity in developing neurons. J. Neuroscience 18: 10749-10761.
Williamson A, Patrylo PR, and Spencer DD (1999) Decrease in inhibition in dentate granule cells from patients with medial temporal lobe epilepsy. Annals of Neurology 45: 92-99.
Patrylo PR, van den Pol AN, Spencer DD, and Williamson A (1999) NPY inhibits glutamatergic excitation in the epileptic human dentate gyrus. J. Neurophysiol. 82: 478-483.
Liu QS, Patrylo PR, Gao XB, and van den Pol AN (1999) Kainate acts at presynaptic receptors to increase GABA release from rat hypothalamic neurons. J. Neurophysiol. 82: 1059-1062.
Patrylo PR, Schweitzer JS and Dudek FE (1999) Abnormal responses to perforant path stimulation in the dentate gyrus of slice from kainate-induced epileptic rats with mossy fiber reorganization. Epilepsy Res. 36: 31-42.
Hellier JL, Patrylo PR, Dou P, Nett M, Rose GM, and Dudek FE (1999) Assessment of inhibition and epileptiform activity in the septal dentate gyrus of freely behaving rats during the first week after kainate treatment. J. Neuroscience 19: 10053-10064.
Williamson A and Patrylo PR (1999) Regulation of excitability in the epileptic human dentate gyrus. The Neuroscientist 5: 362-370.
Dudek FE, Patrylo PR, and Wuarin JP (1999) Mechanisms of neuronal synchronization during epileptiform activity. In: Basic Mechanisms of the Epilepsies. A. Delgado-Esqueta, ed. Adv. Neurol. 79: 699-708.
Patrylo PR, Spencer DD, and Williamson A (2001) GABA uptake and heterotransport are impaired in the dentate gyrus of kainate-induced epileptic rats and humans with temporal lobe sclerosis. J. Neurophysiol. 85: 1533-1542.
van den Pol AN, Patrylo PR, Ghosh PK, and Gao X-B (2001) Lateral hypothalamus: early developmental expression and response to hypocretin (orexin). J. Comp. Neurol. 433: 349-363.
Williamson A, Patrylo PR, Lee S, and Spencer DD (2003) Physiology of human cortical neurons adjacent to cavernous malformations and tumors. Epilepsia 44: 1413-1419.
Horvath TL, Matthews RT, Patrylo, PR, Yang L, Beal MF, Barnstable CJ, and Diano S (2003) Mitochondrial uncoupling protein 2 prevents neuronal death: a mechanism for pre-conditioning. Endocrinology 144: 5014-5021.
Darbin O, Naritoku D, and Patrylo PR (2004) Aging alters electroencephalographic and clinical manifestations of kainate-induced status epilepticus. Epilepsia 45: 1219-1227.
Williamson A, Patrylo PR, Pan J, Spencer DD, and Hetherington H (2005) Correlations between Granule Cell Physiology and Bioenergetics in Human Temporal Lobe Epilepsy. Brain 128: 1199-208.
Leppik IE, Kelly K, de Toledo-Merrell L, Patrylo PR, DeLorenzo RJ, Matthern GW, and White HS (2006) Epilepsy and Aging. Epilepsy Research 68 Suppl 1:21-37.
Patrylo PR, Browning RA, Cranick S (2006) Reeler homozygous mice exhibit enhanced susceptibility to epileptiform activity. Epilepsia 47(2): 1-10.
Kopjas NN, Jones RT, Bany B, Patrylo PR (2006) Reeler mutant mice exhibit seizures during recovery from isoflurane-induced anesthesia. Epilepsy Research 69: 87 -91.
Darbin O, Carre E, Naritoku D, Risso JJ, Lonjon M, Patrylo PR (2006) Glucose metabolites in the striatum of freely-behaving rats following infusion of elevated potassium. Brain Research 1116(1):127-131.
Patrylo PR and Willingham A (2007) Anatomic and electrophysiologic evidence for a pro-convulsive circuit in the dentate gyrus of reeler mutant mice, an animal model of diffuse cortical malformation. Dev. Neuroscience 29: 73 - 83.
Patrylo PR and Williamson A (2007) The effects of aging on dentate circuitry and function. Prog. in Brain Research 163: 679-696.
Williamson A and Patrylo PR (2007) Physiological studies of human dentate granule cells. Prog. in Brain Research 163: 183-209.
Patrylo PR, Tyagi I, Willingham AL, Lee S, Williamson A (2007) Dentate filter function is altered in a pro-epileptic fashion during aging. Epilepsia 48: 1964-1978.
Xiong K, Luo DW, Patrylo P, Luo XG, Struble RG, Clough RW & Yan XX (2008): Doublecortin expressing cells are present in layer II across adult guinea pig cerebral cortex: Partial colocalization with mature interneuron markers. Exp. Neurol., 211: 271-282.
Zhang XM, Cai Y, Cai H, Xiong K, Luo XG, Feng JC, Clough RW, Struble RG, Patrylo PR, Yan XX (2009) BACE1 elevation in transgenic mouse models of Alzheimer's disease is associated with synaptic/axonal pathology and amyloidogenesis: implications for neuritic plaque development. Eur J. Neurosci, 30:2271-2283.
Zhang XM, Cai Y, Chu Y, Chen EY, Feng JC, Luo XG, Xiong K, Struble RG, Clough RW, Patrylo PR, Kordower JH, Yan XX.(2009) Doublecortin-expressing cells persist in the associative cerebral cortex and amygdala in aged nonhuman primates. Front Neuroanat. 3:17.
Cai Y, Xiong K, Chu Y, Luo DW, Luo XG, Yuan XY, Struble RG, Clough RW, Spencer DD, Williamson A, Kordower JH, Patrylo PR, Yan XX. (2009) Doublecortin expression in adult cat and primate cerebral cortex relates to immature neurons that develop into GABAergic subgroups. Exp Neurol. 216(2):342-56.
RG Struble, TA Ala, PR Patrylo, GJ Brewer and Xioa-Xin Yan. (2010) Is Brain Amyloid a cause or a Result of Dementia of the Alzheimer Type? J. of Alzheimer's Disease 22(2): 393-399.
Y Cai, K Xiong, XM Zhang, H Cai, XG Luo, JC Feng, RW Clough, RG Struble, PR Patrylo,Y Chu, JH Kordower and XX Yan. (2010) β-Secretase-1 elevation in aged monkey and Alzheimer's disease human cerebral cortex occurs around vasculature in partnership with multisystem axon terminal pathogenesis and β-amyloid accumulation. Eur J. Neuroscience 32(7): 1223-1238.
XM Zhang, K Xiong, Y Cai, H Cai, XG Luo, JC Feng, RW Clough, PR Patrylo, RG Struble, XX Yan. (2010) Functional deprivation promotes amyloid plaque pathogenesis in Tg2576 mouse olfactory bulb and piriform cortex. Eur J. Neuroscience 31(4): 710-721.
Xiong K, Cai Y, Zhang XM, Huang JF, Liu ZY, Fu GM, Feng JC, Clough RW, Patrylo PR, Luo XG, Hu CH, Yan XX (2010). Layer 1 as a putative neurogenic niche in young adult guinea pig cerebrum. Mol Cell Neurosci. 45(2): 180-191.
Magnusson KR, Das SR, Kronemann D, Bartke A, Patrylo PR. (2011) The effects of aging and genotype on NMDA receptor expression in growth hormone receptor knockout (GHRKO) mice. J Gerontol A Biol Sci Med Sci. Jun;66(6):607-19.
Kanak DJ, Jones RT, Tokhi A, Willingham AL, Zaveri HP, Rose GM, Patrylo PR. (2011) Electrical and Pharmacological Stimuli Reveal a Greater Susceptibility for CA3 Network Excitability in Hippocampal Slices from Aged vs. Adult Fischer 344 Rats. Aging Dis. Aug;2(4):318-31.
Cai Y, Zhang XM, Macklin LN, Cai H, Luo XG, Oddo S, Laferla FM, Struble RG, Rose GM, Patrylo PR, Yan XX. (2012) BACE1 elevation is involved in amyloid plaque development in the triple transgenic model of Alzheimer's disease: differential Aβ antibody labeling of early-onset axon terminal pathology. Neurotox Res. Feb;21(2):160-74.
Yan XX, Cai Y, Zhang XM, Luo XG, Cai H, Rose GM and Patrylo PR. (2012) BACE1 Elevation is Associated with Aberrant Limbic Axonal Sprouting in Epileptic CD1 Mice Exp Neurology235(1):228-237.
Cai Y, Zhu HX, Li JM, Luo XG, Patrylo PR, Rose GM, Streeter J, Hayes R, Wang KK, Yan XX, and Jeromin A. (2012) Age-related intraneuronal accumulation of αII-spectrin breakdown product SBDP-120 in rodent forebrain accelerates in 3xTg-AD mice. PLOs One 7(6):e37599.
Li JM, Xue ZQ, Deng SH, Luo XG, Patrylo PR, Rose GW, Cai H, Cai Y, Yan XX. (2012) Amyloid plaque pathogenesis in 5xFAD mouse spinal cord: retrograde transneuronal modulation after peripheral nerve injury. Neurotox Res. (in press).
Yan XX, Cai Y, Shelton J, Deng SH, Luo XG, Oddo S, Laferla FM, Cai H, Rose GM, Patrylo PR. (2012). Chronic Temporal Lobe Epilepsy Is Associated with Enhanced Alzheimer-Like Neuropathology in 3xTg-AD Mice. PLOs One (in press).
Liu F, Xue ZQ, Deng SH, Kun X, Luo XG, Patrylo PR, Rose GM, Cai H, Struble RG, Cai Y, Yan XX. (2013) γ-Secretase binding sites in aged and Alzheimer's disease human cerebrum: the choroid plexus as a putative origin of CSF Aβ. Eur J Neurosci. 37(10):1714-25.Kanak DJ, Rose GM, Zaveri HP, Patrylo PR. (2013) Altered Network Timing in the CA3-CA1 Circuit of Hippocampal Slices from Aged Mice. PLoS One 8(4):e61364..
Deng X, Li M, Ai W, He L, Lu D, Patrylo PR, Cai H, Luo X, Li, Z, Yan XX (2014) Lipolysaccharide-Induced Neuroinflammation is Associated with Alzheimer’s-Like Amyloidogenic Axonal Pathology and Dendritic Degeneration in Rats. Adv in Alzheimer’s Disease 3(2):78-93.
Li JM, Cai Y, Liu F, Yang, Hu X, Patrylo PR, Cai H, Juo XG, Xiao D, Yan XX (2015) Experimental microembolism induces localized neuritic pathology in guinea pig cerebrum. Oncotarget ;6(13):10772-85.
Jones RT, Tyagi I, Patrylo PR (2015) Preferential reduction of synaptic efficacy in the dentate gyrus of hippocampal slices from aged rats during reduced glucose availability. Neuroscience. 307:262-72.
Yang Y, Xie MX, Li JM, Hu X, Patrylo PR, Luo XG, Cai Y, Li Z, Yan XX. (2015) Prenatal genesis of layer II doublecortin expressing neurons in neonatal and young adult guinea pig cerebral cortex. Front Neuroanat. 9:109.
Zhang XM, Cai Y, Wang F, Wu J, Mou L, Zhang F, Patrylo PR, Pan A, Ma C, Fu J, Yan XX. (2016) Sp8 expression in putative neural progenitor cells in guinea pig and human cerebrum. Dev Neurobiol. 76(9):939-55.
Griffith CM, Xie MX, Qiu WY, Sharp AA, Ma C, Pan A, Yan XX, Patrylo PR (2016) Aberrant expression of the pore-forming KATP channel subunit Kir6.2 in hippocampal reactive astrocytes in the 3xTg-AD mouse model and human Alzheimer's disease. Neuroscience. 336:81-101.
Hayashi-Park E, Ozment BN, Griffith CM, Zhang H, Patrylo PR, Rose GM. (2017) Experimentally induced diabetes worsens neuropathology, but not learning and memory, in middle aged 3xTg mice. Behav Brain Res. 2322(Pt B):280-287.
Macklin L, Griffith CM, Cai Y, Rose GM, Yan XX, Patrylo PR (2017) Glucose tolerance and insulin sensitivity are impaired in APP/PS1 transgenic mice prior to amyloid plaque pathogenesis and cognitive decline. Exp Gerontol. 88:9-18.
Griffith CM, Macklin LN, Bartke AJ, Patrylo PR (2017) Differential Fasting Plasma Glucose and Ketone Body Levels in GHRKO versus 3xTg Mice: A Potential Contributor to Aging-Related Cognitive Status? Int J Endocrinology doi: 10.1155/2017/9684061.
Griffith CM, Eid T, Rose GM, Patrylo PR. (2018) Evidence for altered insulin receptor signaling in Alzheimer's disease. Neuropharmacology. Jul 1;136(Pt B):202-215.
Zhou FQ, Jiang J, Griffith CM, Patrylo PR, Cai H, Chu Y, Yan XX. (2018) Lack of human-like extracellular sortilin neuropathology in transgenic Alzheimer's disease model mice and macaques. Alzheimers Res Ther. Apr 24;10(1):40. doi: 10.1186/s13195-018-0370-2.
Eid T, Lee TW, Patrylo P, Zaveri HP. (2019) Astrocytes and Glutamine Synthetase in Epileptogenesis. J Neurosci Res. Nov;97(11):1345-1362.
Griffith CM, Macklin LN, Cai Y, Sharp AA, Yan XX, Reagan LP, Strader AD, Rose GM, Patrylo PR. (2019) Impaired Glucose Tolerance and Reduced Plasma Insulin Precede Decreased AKT Phosphorylation and GLUT3 Translocation in the Hippocampus of Old 3xTg-AD Mice. J Alzheimers Dis. 68(2):809-837.
Ebersole JM, Rose GM, Eid T, Behar KL, and Patrylo PR. (2021) Altered Hippocampal Astroglial Metabolism is Associated with Aging and Preserved Spatial Learning and Memory. Neurobiol of Aging (in press).
Chapters in Professional Books
Dudek FE, Patrylo PR, and Wuarin JP (1999) Mechanisms of neuronal synchronization during epileptiform activity. In: Basic Mechanisms of the Epilepsies. A. Delgado-Esqueta, ed. Adv. Neurol. 79: 699-708.
Patrylo PR (2009) Aging and Seizure Susceptibility. In: Encyclopedia of Basic Epilepsy Research. PA Schwartzkroin ed. Elsevier First Edition pages 40-45.